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[Effects of anion transport inhibitor 5-(N,N-dimethyl)-amiloride on myocardial mitochondria Ca2+ transport in post-ischemic reperfusion period of isolated rat hearts]. The objective of this study is to investigate whether 5-(N,N-dimethyl)-amiloride (DMA), an inhibitor of anion transport and Na(+)-H+ exchange, can improve the myocardial mitochondrial Ca2+ transport in post-ischemic reperfusion period. The isolated rat hearts with Langendorff perfusion technique were perfused under the condition of normal Ca2+ (2.5 mmol/L) or high Ca2+ (5.0 mmol/L) for 10 min, and subsequently at the different Ca2+ levels after a 30 min period of no flow, followed by a 20 min period of reflow. The [Ca2+]i at various time points after reflow was monitored using the fluorescent Ca2+ probe indo-1. The Ca2+ content of mitochondria was analyzed by using electron probe X-ray microanalyzer (EPMA). The results showed that in normal Ca2+, the levels of [Ca2+]i during reflow were much higher than those before ischemia (P Q: Picking random cards without replacement in Java I'm currently practicing for a CS exam, and I have this problem: You are given a deck of 52 cards, where a card has a unique number. You are allowed
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